3D CELL CULTURE DISH

STEM FIT 3D Adventage

STEM FIT 3D Adventage

  • 01 ADVENTAGE
  • Shorter time consuming to generate
  • spheroids than hanging drop method
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  • 02 ADVENTAGE
  • Uniform size spheroid
  • Secure the Statistical significance advantage
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  • 03 ADVENTAGE
  • Can be used as stem cell treatment
  • transplantation survival rate is higher than single cells
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  • 04 ADVENTAGE
  • All materials which are used at 2D cell culture can be used
  • Can use existing cell culture material
    without additional purchase
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  • 05 ADVENTAGE
  • Co-aggregation & Co-culture with
    reproductive integrity cells
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  • 06 ADVENTAGE
  • Minimum consuming of expensive reagents (antibody , cytokine and chemokine) and Save up limited funds
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How to use STEMFIT 3D

3D Culture Dish

  • weight

    2g

  • Raw Materials

    PDMS (Polydimethylsiloxane)

  • Type

    Concave Microwell

  • Usage

    Disposable


Cat.No
C100600
Product name
StemFIT 3D C100
Number of micro wells
100
Well Diameter
600μm

Cat.No
H389600
Product name
StemFIT 3D H389
Number of micro wells
389
Well Diameter
600μm

Cat.No
H853400
Product name
StemFIT 3D H853
Number of micro wells
853
Well Diameter
400μm

Cat.No
C253000
Product name
StemFIT 3D C25
Number of micro wells
25
Well Diameter
3000μm
  • photo
  • Cat.No
  • Product name
  • Number of micro wells
  • Well Diameter
  • C100600
  • StemFIT 3D C100
  • 100
  • 600μm
  • H389600
  • StemFIT 3D H389
  • 389
  • 600μm
  • H853400
  • StemFIT 3D H853
  • 853
  • 400μm
  • C253000
  • StemFIT 3D C25
  • 25
  • 3000μm

How to use

Watch the video


1-1.

Unpack sterilized StemFit 3D.


1-2.

Position StemFit 3D on dish.


1-3.

Add 70% EtOH into the StemFIT 3D.

2.

Remove ALL bubbles inside of each micro well by pipetting


3.

Suction at the corner of square bottom Of StemFIT 3D. (Just select one Point for suction)

3-1.

Caution – Do not absorb into each Miico well/


4.

Fill up cell culture media and then remove bubbles of inside of Micro wells.


5.

Check out removal of all bubbles Through microscope.


6.

Cell Seeding

7.

Wait until almost cells settle down on the bottom of StemFIT 3D. ( For FIVE minutes )


8.

Gentle suction – each corner of square bottom of StemFIT 3D. FOR ONLY C100600

*

Gentle suction is very critical point. Flow of cell suspension by suction allows cells , out of micro wells , to roll in the each micro well.


9.

Fully fill the StemFIT 3D up with culture media.


10-1.

Incubation at humid environment. *First media change should be don with in 4 to 24 hrs


10-2.

Media change : GENTLY popetting old media from the corner of bottom square. and then add fresh media GENTLY the same spot.


11-1.

Live observation during culture period

11-2.

Harvest formed Spheroids via Hard Pipetting.

Referance list

Review

Trends in Biotechnology“Central Nervous System and its Disease Models on a Chip”

Advanced Healthcare Materials“Bottom-up engineering of well-defined 3D microtissues using microplatforms and biomedical applications”

Lab Chip“3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip”

Stem Cells Translational Medicine“Stem cell microenvironment on a chip: current technologies for tissue engineering and stem cell biology”

Stem cell

Biomaterials, 31, 2010, 4296-4303“Central Nervous System and its Disease Models on a Chip”

Biomaterials, 35, 2014, 5987-5997“Bottom-up engineering of well-defined 3D microtissues using microplatforms and biomedical applications”

Adv. Healthcare Mater., 2013, 2, 119-125“3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip”

Lab Chip, 10, 2010, 2651-2654“Stem cell microenvironment on a chip: current technologies for tissue engineering and stem cell biology”

Biomedical Reports 2015, 97-101“Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests”

Organ regeneration

Biomaterials, 32, 2011, 8087-8096“Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model”

Biomaterials, 33(3), 2012, 837-45“In situ formation and collagen-alginate composite encapsulation of pancreatic islet spheroids”

Biomaterials, 34, 2013, 3784-3794“3D co-culturing model of primary pancreatic islets and hepatocytes in hybrid spheroid to overcome pancreatic cell shortage”

Biomaterials, 35, 2014, 8983-8991“Immune-protected xenogeneic bioartificial livers with liver-specific microarchitecture and hydrogel-encapsulated cells”

Acta Biomaterialia, 35, 2016 215-227“Scaffold-free parathyroid tissue engineering using tonsil-derived mesenchymal stem cells”

CNS & Cancer Model / Screening

Biomaterials, 34, 2013, 2938-2946 “Size-controllable networked neurospheres as a three-dimensional neuronal tissue model for Alzheimer’s disease studies”

Lab Chip., 15, 2015, 141-50 “Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer’s disease”

Nanomedicine, 11, 2015, 1153–1161 “Concave microwell array-mediated three-dimensional tumor model for screening anticancer drug-loaded nanoparticles”

PLoS ONE, 8(9): e73345. doi:10.1371, 2013“Application of Concave Microwells to Pancreatic Tumor Spheroids Enabling Anticancer Drug Evaluation in a Clinically Relevant Drug Resistance Model

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